primary antibody against bmpr1b  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Tunneling nanotubes propagate a BMP-dependent preneoplastic state

doi: 10.1101/2025.11.11.687852

Figure Lengend Snippet: | Actors of the BMP signaling pathway are transferred to acceptor cells through TNTs. a, GOBP subset from a list of 6 protein hits dysregulated between NoTf accept and NoTf no accept cells, generated through STRING from proteins of the BMP and p38 pathways, chosen from the literature. The size of each circle represents the number of genes in the respective category; the intensity of the color represents the P-value, determined by a hypergeometric test. b, Box and whiskers plots of Log 2 LFQ values (Protein Levels) of ID1 ( ID1 ), Nup214 ( NUP214 ), Spartin ( SPART ), ATF2 ( ATF2 ), Rac1 ( RAC1 ) and desmoglein-3 ( DSG3 ), for indicated sorted cell populations. Statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparison test (n = 5 biological replicates; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). For clarity, only comparison between NoTf and NoTf accept cells is shown. c, Immunofluorescence images of actin (red) and BMPR1b (green) in indicated cells. Nuclei (blue). Scale bars, 20 μm. d, Correlative light scanning electron microscopy (SEM) of BMPR1b, immunostained with Alexa Fluor ® 488-Fluoronanogold ™ (green) in M1B26 cells. From left to right: confocal, SEM, zooms, correlative (overlay). Scale bars, 10 μm, unless otherwise indicated. e, Immunofluorescence of actin (green), after transfection with siRNA pools targeting BMPR1b (siBMPR1b) or GPER (siGPER). Non-targeting siRNA: siCTRL. f,g, BMPR1B and GPER transcript (f) and protein expression upon their silencing (f),with siCTRL set at 1 (n=4), and TNT number (> 8 μm) per cell and % of long TNTs (> 50 μm) upon indicated silencing, normalized against siCTRL (g). Error bars depict SD. Statistical significance was calculated using a Student’s two-sided t test with Welch’s correction (n = 4 biological replicates, *** p < 0.001). h, Schematic of experimental design: after sorting, cell populations were either lysed and subjected to RT-qPCR for selected transcript analyses, or grown for several weeks with or without BMP2/IL6, and immunoblot analyses performed at end points. i, RT-qPCR measurement of BMPR1b mRNA in indicated cell populations, the expression in NoTf cells set at 1. Statistical significance was calculated using one-way ANOVA with post-hoc Tukey test (n = 5 biological replicates). For clarity, only comparison between NoTf accept and NoTf cells is shown (**** p < 0.0001).

Article Snippet: After PBS washing, primary antibody against BMPR1b (1:300 #sc-293428 Santa Cruz) and its corresponding isotype were incubated overnight at 4°C.

Techniques: Generated, Comparison, Immunofluorescence, Electron Microscopy, Transfection, Expressing, Quantitative RT-PCR, Western Blot

Journal: bioRxiv

Article Title: Tunneling nanotubes propagate a BMP-dependent preneoplastic state

doi: 10.1101/2025.11.11.687852

Figure Lengend Snippet: | Non-transformed cells acceptor of information via TNTs from transformed cells display preneoplastic features. a, Left, quantification of BMPR1b immunoblots performed on NoTf accept cells grown in the presence of BMP2/IL6, for indicated weeks post sorting. Box and whiskers plot of 6 biological replicates. Statistical significance was calculated using a one-way ANOVA with non-parametric Kruskal-Wallis test, and for clarity, only p-values ≤ 0.05 are indicated. Right, quantification of BMPR1b immunoblots performed on indicated cells, 14 weeks post sorting, with NoTf conditions set at 1 (n = 6 biological replicates). Statistical significance was calculated using a one-way ANOVA with post-hoc Tukey test (*** p < 0.001). b,c, Quantification of p-SMAD1/5/8/SMAD1/5/8 (b) and p-p38/p38 (c) immunoblots performed on NoTf accept cells grown in the presence of BMP2/IL6, for indicated weeks post sorting. Box and whiskers plot of 6 biological replicates. Statistical significance was calculated using a one-way ANOVA with non-parametric Kruskal-Wallis test, and for clarity, only p-values ≤ 0.05 are indicated. d, RNA-sequencing results of transcriptomic expression levels of HOXB6, CCND2, EREG and HRAS in indicated cell lines (n = 3 biological replicates). Data deposited on the Gene Expression Omnibus repository, GSE186734 . Error bars depict SD. Statistical significance was calculated using a one-way ANOVA with post-hoc Tukey test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). e, RT-qPCR measurements of HOXB6, CCND2, EREG and HRAS mRNA in indicated cell populations, with the expression in NoTf cells set at to 1. Error bars depict SD. Statistical significance was calculated using a one-way ANOVA with post-hoc Tukey test (Biological replicates: n = 8 for HOXB6, n = 5 for CCND2, n = 7 for EREG, n = 8 for HRAS). For clarity, only comparison between NoTf accept and NoTf cells is shown (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). f, Schematic of long-term soft agar colony formation assays. g, Quantification of soft agar clones of indicated sorted cells, after 16 weeks in culture with or without BMP2/IL6 (n = 3 biological replicates). Error bars represent SD. Statistical significance was calculated using a one-way Welch and Brown-Forsythe ANOVA test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). For clarity, only p-values ≤ 0.05 are indicated, and comparisons with Tf cells are omitted. h, Model depicting how TNTs are involved in the propagation of a BMP-dependent preneoplastic state. Fibrils and fibers depict extracellular matrix components.

Article Snippet: After PBS washing, primary antibody against BMPR1b (1:300 #sc-293428 Santa Cruz) and its corresponding isotype were incubated overnight at 4°C.

Techniques: Transformation Assay, Western Blot, RNA Sequencing, Expressing, Gene Expression, Quantitative RT-PCR, Comparison, Clone Assay

Journal: Biology

Article Title: BMP-2-Driven Osteogenesis: A Comparative Analysis of Porcine BMSCs and ASCs and the Role of TGF-β and FGF Signaling.

doi: 10.3390/biology14060610

Figure Lengend Snippet: Figure 6. Receptor expressions of ALK2, ALK4, ALK6, ALK5, ALK7, BMPR-II in pASC. Grouped representation of the respective receptor expression in the course of osteogenic differentiation (OM +/−BMP-2). From day 19, there is a significant induction of ALK 2, ALK 6, and ALK 5 with the addition of BMP-2. BMPR-II expression in the OM group decreased in OM and tended to stay increased under BMP-2 supplementation from day 19, but was not considered significant (* p ≤0.05, ** p ≤0.01; n = 6, BMP-2 450 ng/mL).

Article Snippet: The respective conjugated antibodies were used for the expressions of ALK3 (Cat. No.: AF436), ALK 5 (Cat. No.: FAB5871), ALK6 (Cat. No.: FAB5051A), TGF-β2-RII (Cat. No.: FAB532P), ALK7 (Cat. No.: FAB77491A), ALK2 (Cat. No.: AF637), ALK4 (Cat. No.: MAB2221), and BMPR-II (Cat. No.: AF811) (by R&D Systems, Minneapolis, MN, USA), and the pASCs and pBMSCs were compared for their expressions of the specific surface antigens CD45 (Cat. No.: MCA1568GA, BioRad, Hercules, CA, USA), HLA-DR (human leukocyte antigen–antigen D-related surface molecule) (Cat. No.: MCA2314F, Bio-Rad, Hercules, CA, USA), CD29 (Cat. No.: 561,496, BD Pharmingen, Franklin Lakes, NJ, USA), CD79alpha (Bio-Rad, Cat. No.: MCA2538GA), CD14 (Cat. No.: MCA1568GA, Bio-Rad, Hercules, CA, USA), CD31 (Cat. No.: AF3387, R&D Systems, Minneapolis, MN, USA), CD105 (Cat. No.: NB110-58718APC, Novus Biologicals, Minneapolis, MN, USA), CD26 (, Cat. No.: NB600-552APC, Novus Biologicals, Minneapolis, MN, USA), CD73 (, Cat. No.: AF4488, R&D Systems, Minneapolis, MN, USA), CD90 (Cat. No.: 559,869, BD Pharmingen, Franklin Lakes, NJ, USA), CD34 (Cat. No.: 81289, abcam, Cambridge, UK), and CD44 (Cat. No.: 5531, BD Pharmingen, Franklin Lakes, NJ, USA).

Techniques: Expressing